Rat hepatocyte spheroids formed by rocked technique maintain differentiated hepatocyte gene expression and function
نویسندگان
چکیده
منابع مشابه
Rat hepatocyte spheroids formed on temperature-responsive PIPAAm polymer-grafted surface maintain long-term differentiated hepatocyte function
Introduction The development of effective culture methods is very important in regenerative medicine. This is because they may be able to contribute to the advancement of medicine. Since the liver plays an important and complex metabolic role, the development of a practicable culture method to maintain long-term hepatocyte function is highly desirable. However, hepatocytes lose their liver-spec...
متن کاملTranscriptional induction of heme oxygenase-1 gene expression by okadaic acid in primary rat hepatocyte cultures.
Heme oxygenase (HO) catalyzes the rate-limiting enzymatic step of heme degradation and regulates the cellular heme content. The gene expression of the inducible isoform of HO, HO-1, is up-regulated in response to various agents causing oxidative stress. To investigate the regulatory role of protein phosphatases in the hepatic regulation of HO-1 gene expression, primary cultures of rat hepatocyt...
متن کاملHepatocyte nuclear factor 3beta (Foxa2) is dispensable for maintaining the differentiated state of the adult hepatocyte.
Liver-specific gene expression is controlled by a heterogeneous group of hepatocyte-enriched transcription factors. One of these, the winged helix transcription factor hepatocyte nuclear factor 3beta (HNF3beta or Foxa2) is essential for multiple stages of embryonic development. Recently, HNF3beta has been shown to be an important regulator of other hepatocyte-enriched transcription factors as w...
متن کاملEffects of rapamycin on cultured hepatocyte proliferation and gene expression.
Rapamycin, a potent immunosuppressive drug that disrupts normal signal-transduction processes, inhibited hepatocyte proliferation without evidence of inherent cytotoxicity in rat hepatocytes cultured in conventional medium or in a medium enriched with epidermal growth factor. The antiproliferative effect was dose dependent, uninfluenced by the concentration of epidermal growth factor in the med...
متن کاملIL-1β and IL-6 modulate apolipoprotein E gene expression in rat hepatocyte primary culture
Incubation of rat hepatocytes in primary culture with IL-1beta at a concentration of 2.5 units/ml resulted in an increase (+80%) in the amount of apoE mRNA without any effect upon apoE synthesis. IL-6 at a low concentration (10 units/ml) induced a decrease (-35%) in the amount of apoE mRNA, but increased apoE synthesis (+28%). No effect was observed with higher concentrations of IL-1beta (10 un...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Hepatology
سال: 2008
ISSN: 0270-9139
DOI: 10.1002/hep.22674